Invitrogen™ RT1.Bu Monoclonal Antibody (OX-3), PE

Beschreibung

anti-rat RT1.Bu monoclonal antibody recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, DA, or PVG/c strains. This antibody also cross-reacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9. Recognizes Ia antigens on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes. Thus, this antibody is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras.

RT1.Bu is a gene located within the major histocompatibility complex (MHC) region of the rat genome, specifically part of the RT1 complex. The RT1 complex in rats is analogous to the human HLA and mouse H2 MHC regions and encompasses a variety of class I and class II genes involved in antigen presentation. RT1.Bu is one such class I MHC gene, encoding a protein essential for the presentation of endogenous peptides to cytotoxic T cells. This interaction is vital for the immune system's ability to recognize and eliminate infected or malignantly transformed cells. The RT1.Bu gene is crucial for studying immune responses, particularly in transplantation immunology and autoimmune disease models, due to its role in histocompatibility and immune regulation.