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Invitrogen™ Mouse Eotaxin-1 (CCL11) ELISA Kit
ELISA
Marke: Invitrogen™ EMCCL11X5
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Beschreibung
The Mouse Eotaxin-1/CCL11 ELISA quantitates Ms Eotaxin-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Eotaxin-1. Principle of the method The Mouse Eotaxin-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
AHC6054CU erkennt natürliches und rekombinantes Eotaxin.
Spezifikation
| P48298 | |
| 1 pg/mL | |
| Festphasen-Sandwich-ELISA | |
| ELISA-Kit | |
| Plasma, Serum, Überstand | |
| 5 x 96 Assays | |
| ELISA, Protein-Assays, Proteinbiologie, Krebsbiologie, Immunologie, Neurowissenschaften, Neurobiologie, Proteinnachweis, Proteinanalyse | |
| 20292 | |
| <12% | |
| Standard, Vorbeschichtete 96-Well-Platten, Assay-Verdünnungsmittel-Konzentrat, Stopplösung, Dichtungsfolien für Mikrotiterplatten, SAV-HRP, Chromogen, Waschpuffer, Biotinylierter Nachweisantikörper | |
| 5 x 96 Tests | |
| Entzündungen | |
| 2 bis 8 °C | |
| Maus | |
| 4 h 45 min |
| 1,02 pg/mL bis 100 pg/mL | |
| 1 pg/mL bis 100 pg/mL | |
| Biotin | |
| Chemokin und Rezeptoren | |
| Dieses ELISA-Kit weist keine Kreuzreaktivität mit einem der getesteten Zytokine auf: Maus CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin-2, Fas Ligand, Fraktalkin, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN (OB), LIX, L-Selektin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selektin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF) | |
| Maus | |
| Kolorimetrisches Mikrotiterplatten-Lesegerät | |
| Scya11, Eotaxin | |
| <10% | |
| HRP | |
| RUO | |
| Plasma, 10 μl; Serum, 10 μl; Überstand, 100 μl | |
| Scya11, Eotaxin | |
| 1 h 20 min |
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