Invitrogen™ ELISA-Kit für humanes MMP1

Beschreibung

The Human Matrix MetalloProteinase 1 (Hu MMP1) ELISA quantitates Hu MMP1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MMP1. Principle of the method The Human MMP1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Die Matrix-Metalloproteasen (MMP) gehören zu den proteolytischen Enzymen, die in der Lage sind, Bestandteile des Bindegewebes abzubauen. Eine gestörte Sekretion bestimmter MMPs oder Störungen der differentiellen Regulierung von MMPs durch Gewebeinhibitoren der Metalloproteinasen (TIMPs) werden für das invasive Potenzial maligner Tumore verantwortlich gemacht. Interstitielle Kollagenase (MMP-1), 72 kDa-Gelatinase A/Typ IV-Kollagenase (MMP-2) und 92 kDa-Gelatinase B/Typ IV-Kollagenase (MMP-9) lösen extrazelluläre Matrix (ECM) auf und können Angiogenese initiieren und fördern.