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Invitrogen™ EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays
Detects metallo-, serine, acid, and sulfhydryl proteases
Brand: Invitrogen™ E6639
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Description
The EnzChek Protease Assay Kit, red fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with a fluorescence microplate reader, is proportional to protease activity. This EnzChek Protease Assay Kit contains a casein derivative that has been extensively labeled with the red-fluorescent BODIPY TR-X dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY TR-X dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The red-fluorescent BODIPY TR-X dye has excitation and emission spectra similar to those of the Texas Red fluorophore.
- Generalized mechanism allows for measurement of variety of proteases
- Can be used to continuously measure protease kinetics
- Excitation/emission similar to Texas Red™ dye (589/617nm)
Enzyme and Protein Activity Assays, Protease/Peptidase Activity, Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Room temperature
Specifications
| Store in freezer -5°C to -30°C and protect from light. | |
| Protease Assay | |
| Protease Assay | |
| 1 kit | |
| Protein-Based Substrate | |
| Proteases |
| Fluorescence Intensity | |
| Fluorescence Microplate Reader | |
| EnzChek | |
| Room Temperature | |
| Protease Substrate |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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