Invitrogen™ ANGPTL3 Mouse ELISA-Kit

Beschreibung

The Mouse ANGPTL3 ELISA quantitates Ms ANGPTL3 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms ANGPTL3. Principle of the method The Mouse ANGPLT3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Dieses Gen codiert ein Mitglied einer Familie von sezernierten Proteinen, die in der Angiogenese involviert sind. Das codierte Protein, das überwiegend in der Leber exprimiert wird, wird zu einer N-terminalen Kette mit Coiled-Coil-Domänen und einer C-terminalen Fibrinogen-Kette weiterverarbeitet. Die N-terminale Kette ist für den Lipidstoffwechsel wichtig, während die C-terminale Kette an der Angiogenese beteiligt sein kann. Mutationen in diesem Gen führen zu familiärer Hypobetalipoproteinämie Typ 2. [bereitgestellt von RefSeq, Aug 2015].