Invitrogen™ Mouse Axl ELISA Kit

Beschreibung

The Mouse Axl ELISA quantitates Ms Axl in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Axl. Principle of the method The Mouse Axl solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

AXL (auch Tyrosin-Protein-Kinase-Rezeptor UFO genannt) ist ein Zelloberflächenrezeptor. Der AXL-Rezeptor wandelt Signale aus der extrazellulären Matrix in das Zytoplasma um, indem er Wachstumsfaktoren wie das Vitamin K-abhängige Proteinwachstums-arrestspezifische Gen 6 (GAS6) bindet. Er ist an der Stimulation der Zellvermehrung beteiligt. Dieser Rezeptor kann auch die Zellaggregation durch homophile Bindung vermitteln. AXL ist ein wesentlicher epithelialer bis meschymaler übergangsinduzierter Regulator der Brustkrebsmetastasierung.