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OPTIZYME™ NdeI, Fisher BioReagents™

Brand:  Fisher BioReagents BP8020-1

Product Code. 11964782

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Description

Description

5'...C A^T A T G...3'
3'...G T A T^A C...5'

Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 0 - 20%
  • Buffer 3: 100%
  • Buffer 4: 0 - 20%
  • Buffer 5: 50 - 100%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with NdeI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of NdeI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Compatible Ends: Csp6I, FspBI, Tru1I, VspI
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - no effect
    • 5'...TGm6A(N)4CATA TGCT...3'
  • 3'...AC T(N)4GTATm6ACGA...5'
  • (No effect on cleavage)

    • TRUSTED_SUSTAINABILITY
    Specifications

    Specifications

    10 U/μL
    7.4
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    1500 U
    NdeI
    25 to 50% Water, >50% Glycerin
    >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Nde I
    Keep container tightly closed
    CA.TATG
    Liquid
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    Safety and Handling

    Product Identifier
    • OPTIZYME

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