Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Streptavidin-Kits

Beschreibung

Mit Thermo Scientific™ SMART Digest™ Immunaffinity (IA) Streptavidin-Kits erhalten Sie eine schnelle, einfache, hochgradig reproduzierbare und empfindliche Immunocapture und Proteinverdau in einem einzigen Well. Diese Kits wurden entwickelt, um die Herausforderungen zu beseitigen, die häufig mit dem Prozess der Biomarker-Quantifizierung verbunden sind, indem sie einen einfachen und anwenderfreundlichen Capture/Verdauungs-Workflow bieten, der nur Stunden statt über Nacht dauert. Das Kit reinigt verschiedene Target-Biomoleküle durch die Bildung einer starken Streptavidin-Biotin-Bindung.

Solving the Challenges of Low-level Quantitation in Complex Matrices

The need to improve the ability to identify low-level biomarkers is forcing the analytical community to deal with extremely high levels of analyte and sample complexity, which makes quantitation challenging.

As these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is typically employed to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time consuming, multistep, and prone to irreproducibility.

Immunocapture and Protein Digestion in a Single Well

These IA kits deliver a process that is:

• Fast
• Simple
• Highly reproducible
• Sensitive

Unique Design Reduces Workflow to Hours

In SMART Digest IA Kits, the immunocapture reagents and the temperature-activated, thermally stable trypsin are co-immobilized onto a resin. Following enrichment, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions, resulting in a workflow which takes hours rather than overnight.